According to the literature, chromogenic medium is a new type of medium for detecting microorganisms by using the principle that the enzyme produced by the metabolism of the microorganism itself reacts with the corresponding chromogenic substrate to develop color. These corresponding chromogenic substrates are composed of chromogenic genes and microbial partially metabolizable substances. Under the action of specific enzymes, the chromogenic genes are released to show a certain color, and the color of the colonies can be directly observed to identify the strains. It is a new type of separation medium. The chromogenic medium is used for the screening and separation of microorganisms, and the sensitivity and specificity of the reaction are much better than the traditional medium. So what is the storage requirement for the chromogenic medium during the winter season? Let's follow Xiaobian together!
First: Commercialized media should be stored according to the requirements in the instruction manual. The media label should be marked with the batch number, date of manufacture, expiration date and relevant characteristics of the medium. The preservation and transport conditions used should minimize the loss of moisture and provide mechanical protection.
Second: The prepared medium should be stored at 2 to 25 ° C in the dark. If stored in a non-closed container, it is generally used within three weeks; if stored in a closed container, it is generally used within one year, and the agar medium should not be at 0 ° C or 0.
Store below °C to prevent freezing damage to gel properties. If the medium is to be stored for a long period of time, the agar plates should be sealed or placed in a closed container to prevent moisture loss.
The third type: the medium should be taken out immediately after sterilization, and should not be stored in the high pressure bacteria to avoid affecting the quality of the medium. The prepared medium should be stored at 2 to 25 ° C in the dark.
Fourth: Store in a closed container to prevent moisture loss and prolong storage time.
The fifth type: the remelting after solid medium sterilization should be carried out in a heated water bath or using circulating steam. Only once, the melted medium should be placed in a water bath of 45-50 °C, no more than 8 hours.
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